MLF1IP (phospho T78) polyclonal antibody
产品名称： MLF1IP (phospho T78) polyclonal antibody
英文名称： MLF1IP (phospho T78) polyclonal antibody
产品编号： PAB10333
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MLF1IP.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding T78 of human MLF1IP.
- Host:
- Rabbit
- Reactivity:
- Bovine, Chimpanzee, Dog, Human, Mouse, Rat
- Specificity:
- This antibody is specific to human MLF1IP protein phosphorylated at Thr78.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:5000-1:25000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Immunohistochemistry (20 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation.
Kang YH, Park JE, Yu LR, Soung NK, Yun SM, Bang JK, Seong YS, Yu H, Garfield S, Veenstra TD, Lee KS.Mol Cell. 2006 Nov 3;24(3):409-22.
- Applications
- Western Blot (Transfected lysate)
- Western blot using MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) shows detection of MLF1IP phosphorylated at Thr78.
HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1.
Blots were probed with the MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333), stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
- Entrez GeneID:
- 79682
- Protein Accession#:
- NP_078905;Q71F23
- Gene Name:
- MLF1IP
- Gene Alias:
- CENP-50,CENP-U,CENP-U(50),CENP50,CENPU,FLJ23468,KLIP1,PBIP1
- Gene Description:
- MLF1 interacting protein
- Omim ID:
- 611511
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
- Other Designations:
- KSHV latent nuclear antigen interacting protein 1,centromere protein U,polo-box-interacting protein 1